红芪水提浓缩液对快速老化小鼠学习记忆和单胺类神经递质的影响

摘要：目的 观察红芪水提浓缩液(HRCF)对快速老化小鼠学习记忆能力的改善作用和对脑组织内单胺类神经递质的影响,探讨其改善小鼠学习和记忆能力的作用机制。方法 将老化小鼠随机分为抗快速老化亚系1(SAMR1)对照组、快速老化亚系8(SAMP8)模型组、HRCF组和安理申组,每组20只,持续灌胃给药3个月。给药结束后,通过Morris水迷宫测试各组小鼠学习和记忆成绩的变化,采用高效液相色谱法检测各组小鼠脑组织中去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)及5-羟吲哚乙酸(5-HIAA)含量的变化。结果 模型组快速老化小鼠隐蔽平台的逃避潜伏期明显延长,脑组织中NE、DA、5-HT及5-HIAA含量显著降低(P＜0.01)。与模型组比较,HRCF组小鼠隐蔽平台的逃避潜伏期明显缩短,脑组织中NE、DA、5-HT及5-HIAA含量明显增加,差异有统计学意义(P＜0.05,P＜0.01)。结论 HRCF能够改善痴呆模型小鼠的学习和记忆能力,其机制可能与调节脑组织内单胺类神经递质的含量有关。

关键词：红芪水提浓缩液；快速老化小鼠；迷宫学习；单胺类神经递质

DOI：10.3969/j.issn.1005-5304.2013.08.013

中图分类号：R285.5

文献标识码：A

文章编号：1005-5304(2013)08-0036-04Influence of Hedysari Radix Concentrations Fluid on Learning and Memory and Monoamine Neurotransmitters in Senescence Accelerated Mice LIU Hui-lan1, ZHANG Jun-ying2, WEI Dong-feng3 (1.The Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing 100029, China；2.National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China；3.Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China)

Abstract：Objective To explore the influence of Hedysari radix concentrations fluid (HRCF) on learning and memory and its effect on cerebral monoamine neurotransmitters in senescence accelerated mice. Methods The senescence accelerated mice were randomly divided into the senescence accelerated mouse/resistance 1 (SAMR1) control group, senescence accelerated mouse/prone-8 (SAMP8) model group, HRCF treatment group and aricept treatment group. After 3 months of intragastrical treatment, the learning and memory ability changes and content of norepinephrine, dopamine, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid were detected respectively using Morris water maze and high performance liquid chromatography. Results In the model group, the hidden platform test indicated that the latency to find the hidden platform was remarkably prolonged, and the content of norepinephrine, dopamine, 5-hydroxytryptamine as well as 5-hydroxyindoleacetic acid were significantly reduced (P＜0.01). Compared with the model group, the latency of hidden platform test was remarkably shortened (P＜0.05) and the content of norepinephrine, dopamine, 5-hydroxytryptamine as well as 5-hydroxyindoleacetic acid were obviously increased in HRCF treatment group (P＜0.01). Conclusion HRCF could improve learning and memory ability of SAMP8 and the influence may related to increasing the content of cerebral monoamine neurotransmitters.

Key words：Hedysari radix concentrations fluid；senescence accelerated mice；maze learning；monoamine neurotransmitters

随着我国人口老龄化的出现,老年性痴呆(Alzheimer disease,AD)患者数量显著增加,发病率约占老年人口的7.3%,给个人、家庭和社会带来沉重的负担[1-2]。因此,积极开展防治基金项目：国家科技重大专项-重大新药创制(2013ZX09103002- 002)；北京中医药大学自主选题项目(2009-X-64)AD的药物研究具有重要的医学价值和社会意义。红芪为黄芪中皮为红棕色的一种,与黄芪同科不同属,具有益气固表、利尿脱毒、敛疮生肌等功效[3]。现代药理研究表明,红芪水提物具有抗衰老、抗氧化、抗炎、保护神经、调节机体免疫及抗肿瘤等药理作用[4]。本实验以快速老化小鼠SAMP8为AD动物模型,采用Morris水迷宫和高效液相-电化学的方法研究红芪水提浓缩液(HRCF)对快速老化小鼠学习记忆功能和脑组织内单胺类神经递质含量的影响,探讨HRCF改善小鼠学习和记忆能力的作用机制。

相关热词搜索： 小鼠 老化 神经 记忆 快速