不同养殖方式对鳙肠道细菌群落结构的影响

文章编号：2095-1191（2017）05-0907-06

Effects of different aquaculture models on intestinal bacterial

community of bighead carp（Aristichthys nobilis）

XIA Yun1，2， YU De-guang 1， XIE Jun1*， WANG Guang-jun1， YU Er-meng 1， XIE Rui-tao 2

（1 Pearl River Fisheries Research Institute， Chinese Academy of Fishery Sciences / Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation， Ministry of Agriculture， Guangzhou 510380， China； 2 Fisheries College， Guangdong Ocean University， Zhanjiang， Guangdong 524025， China）

Abstract：【Objective】The present study was carried out to evaluate the effects of different aquaculture models on intestinal bacteria composition and diversity of Aristichthys nobilis， to provide reference for healthy culture of it. 【Method】A feeding trial was carried out for eight weeks， and was divided into three groups： biofloc group， fertilization group and cage hanging group. The intestinal colonization bacteria of A. nobilis which were cultivated in three different models was compared by PCR-DGGE technology. 【Result】Results showed that the order of intestinal bacteria diversity followed as biofloc group>fertilization group>cage hanging group， and the DGGE map similarities between biofloc group and fertilization group， biofloc group and cage hanging group， fertilization group and cage hanging group were 53.8%， 39.4% and 42.8% respectively. Specific bands in biofloc group represented Gluconacetobacter， Staphylococcus， Chloroflexi and some uncultured bacteria（EU585886.1， FN824844.1， GU498473.1， GU486235.1 and JN399992.1） of α-subset. Specific bands in fertilization group and cage hanging group were Clostridium， Aeromonas and uncultured bacteria（EU376178.1）. 【Conclusion】The application of biofloc enhances the diversity of intestinal microflora composition in A. nobilis， and reduces the distribution of Aeromonas in A. nobilis intestine. Biofloc can also be used as one kind of high protein bait for A. nobilis.

相关热词搜索： 群落 肠道 细菌 养殖 结构