文章编号：2095-1191（2019）06-1256-07
Abstract：【Objective】The microorganisms which could effectively degrade picoxystrobin were selected and their degrading characteristics were analyzed. It would provide new resources for microbial remediation of methoxy acrylates pesticide residue such as picoxystrobin. 【Method】Picoxystrobin degrading strains were isolated by eichment culture， and the degrading strains were analyzed and identified through physiological， biochemical characteristics and phylogenetic analysis of 16S rRNA gene sequences. Residue of picoxystrobin was quantified by gas chromatograph（HPLC）， and the degrading characteristics were also studied. Metabolics of picoxystrobin in degrading process were identified by gas chromatograph-mass spectrometer（GCMS）， and metabolic pathways of degrading process of the strain on picoxystrobin were analyzed. 【Result】A degrading strain PY3 which took picoxystrobin as the only carbon source was isolated. Physiological， biochemical characteristics and phylogenetic analysis of 16S rRNA sequences indicated that strain PY3 was Rhodopseudomonas palustris. The optical growth and degrading picoxystrobin conditions of strain PY3 were pH 6.0 and 35 ℃. Under the optimal degradation conditions， up to 72.0% of 50 mg/L picoxystrobin could be degraded by strain PY3 in 11 d. Metabolic pathways of picoxystrobin degraded by strain PY3 included oxygen bridge bond between benzene ring and N-containing heterocyclic ring braking and ring-opening reaction of benzene ring and N-containing heterocyclic ring. 【Conclusion】R. palustris PY3 has excellent picoxystrobin degradation capacity and broad pH and temperature tolerance. It can be applied to repair of methoxy acrylates pesticide residues such as picoxystrobin in field eco-environment.
Key words： picoxystrobin; isolation; identification; Rhodopseudomonas palustris; degrading characteristics
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